PND-SNAD工艺处理污泥消化液实际工程启动研究

Study of start-up of full scale PND-SNAD project for treating sludge digestion liquor

中文关键词: 污泥消化液  部分亚硝化-反硝化(PND)  同步亚硝化-厌氧氨氧化-反硝化(SNAD)  脱氮除碳  微生物群落

英文关键词: sludge digestion liquor  partial nitritation and denitrification (PND)  simultaneous nitritation-anammox-denitrification (SNAD)  nitrogen and carbon elimination  microbial community

中文摘要:

      针对同步亚硝化-厌氧氨氧化-反硝化(SNAD)工艺在实际工程应用中易受水质、水量变化冲击的问题，构建了部分亚硝化-反硝化(PND)与SNAD组合工艺处理污泥消化液．在197 d的连续运行过程中，逐级增加水量，最终实现了400 m3/d的设计处理水量，出水COD和TN浓度分别为421.1和330.9 mg/L，出水COD和TN去除率分别为80.3%和88.0%，PND-SNAD工艺成功启动．碳氮平衡分析表明，PND单元对COD和TN的去除贡献率分别为65.9%和42.6%，出水NH4+-N浓度降低至514.0 mg/L，有效改善了SNAD单元的进水水质．SNAD单元中，82.1%的TN通过厌氧氨氧化途径去除．高通量测序结果表明，PND单元絮状污泥中氨氧化菌相对丰度(0.194%)显著高于反硝化菌相对丰度(0.063%)，有利于实现亚硝化． Candidatus Brocadia 是优势厌氧氨氧化菌，在SNAD单元生物膜上显著富集(相对丰度为22.44%)．

英文摘要:

      In response to the challenges faced by the simultaneous nitritation-anammox-denitrification (SNAD) process in practical engineering applications, including susceptibility to fluctuations in water quality and quantity, a combined process of partial nitritation and denitrification (PND) with SNAD is constructed for treating sludge digestion liquor. During the continuous 197-day operation, the water volume is gradually increased, ultimately reaching a design capacity of 400 m3/d. The effluent concentrations of COD and TN are 421.1 and 330.9 mg/L, with COD and TN removal efficiencies of 80.3% and 88.0%, respectively. The PND-SNAD process is successfully started. Carbon and nitrogen balance analysis reveals that the PND unit contributes 65.9% to COD removal and 42.6% to TN removal, reducing the effluent NH4+ -N concentration to 514.0 mg/L, which effectively improves the influent quality to the SNAD unit. In the SNAD unit, 82.1% of TN is removed through the anammox pathway. High-throughput sequencing results reveal that the relative abundance of ammonia-oxidizing bacteria (0.194%) in the PND unit′s flocculent sludge is significantly higher than that of denitrifying bacteria (0.063%), which favors nitritation. Candidatus Brocadiais the dominant anammox bacterium, significantly enriched on the biofilm of the SNAD unit (relative abundance of 22.44%).