文章摘要
对苯二胺类抗氧化剂的体外代谢途径研究
Metabolic Pathways and Products of TireRubber p-Phenylenediamine Antioxidants in Human Liver Microsomes
投稿时间:2024-04-09  修订日期:2024-05-09
DOI:
中文关键词: 对苯二胺抗氧化剂  人肝微粒体  体外代谢  产物鉴定  毒性预测
英文关键词: p-phenylenediamine antioxidants  human liver microsomes  in vitro metabolism  metabolites identification  toxicity prediction
基金项目:
作者单位
宋泽华 工业生态与环境工程教育部重点实验室 
傅志强* 工业生态与环境工程教育部重点实验室 
陈景文 工业生态与环境工程教育部重点实验室 
摘要点击次数: 18
全文下载次数: 0
中文摘要:
      轮胎橡胶抗氧化剂对苯二胺(PPDs)是一类普遍存在的新污染物,因其在环境中被转化为高毒性醌类产物(PPDQs)而受到关注。然而,PPDs在人体内的转化途径和产物尚不清楚,限制了对其环境风险的准确认识。采用人肝微粒体孵育结合液相色谱-串联质谱检测,研究了三种PPDs (N-异丙基-N′-苯基对苯二胺IPPD、N-环己基-N′-苯基对苯二胺CPPD和N,N′-二苯基对苯二胺DPPD)的I相代谢过程。共鉴定得到47种代谢产物,脱氢、羟基化和N-脱烷基是主要的代谢途径。发现三种PPDs均能转化为PPDQs,苯基取代的DPPD比烷基取代的IPPD和CPPD的代谢产物种类少,但相对丰度高。毒性预测结果表明,脱氢、羟基化等主要产物具有潜在的致癌性。研究结果提供了PPDs人体代谢归趋的基础数据,有望用于PPDs的健康风险评价。
英文摘要:
      P-phenylenediamines (PPDs), a type of antioxidants used in tire rubber, are emerging pollutants of concern due to their transformation into highly toxic quinone products (PPDQs) in the environment. However, the metabolic pathways and products of PPDs in organisms are still unclear, hindering the accurate understanding of their environmental risks. This study investigated the phase I metabolism of three PPDs (N-isopropyl-N′-phenyl-p-phenylenediamine (IPPD), N-cyclohexyl-N′-phenyl-p-phenylenediamine (CPPD), and N, N′-diphenyl-p-phenylenediamine (DPPD) using human liver microsomes combined with Liquid Chromatograph-Mass Spectrometer. A total of 47 metabolites were identified, with dehydrogenation, hydroxylation, and N-dealkylation being the main metabolic pathways. It was found that all three PPDs could be transformed into PPDQs, and the phenyl-substituted DPPD possessing fewer but more abundant metabolites than alkylated IPPD and CPPD. Toxicity prediction results indicated that the main products such as dehydrogenation and hydroxylation metabolites have potential carcinogenicity. The results provide basic data for the metabolic fate and toxicity of PPDs in humans, thus benefits the health risk assessment of PPDs.
View Fulltext   查看/发表评论  下载PDF阅读器
关闭